Hello friends, in today, we see the different types of mcqs of Gas Chromatography technique in biotechnolgy. so let's begin
Gas Chromatography technique in biotechnology:
1.
For the separation of which of the following
substances, Gas-solid chromatography is being used?
a) Thermally stable organic components
b) Volatile organic components
c) Thermally stable inorganic components
d) Low molecular weight gaseous species
2.
Which of
the following is not a feature of carrier gas used in gas chromatography?
a) It must be chemically inert
b) It should be suitable for the detector employed
c) It should not be completely pure
d) It should be cheap
3.
Which of the following is the disadvantage of
hydrogen, which can be used as carrier gas in gas chromatography?
a) Dangerous to use
b) Expensive
c) Reduced sensitivity
d) High density
4.
Which of the following is the disadvantage of
helium, which can be used as carrier gas in gas chromatography?
a) Dangerous to use
b) Expensive
c) Reduced sensitivity
d) High density
5.
Slow injection of large samples leads to band
broadening and loss of resolution.
a) True
b) False
6.
In which of the following methods are liquid
samples injected into the column in gas chromatography?
a) Gas tight syringe
b) Micro-syringe
c) Rotary sample valve
d) Solid injection syringes
7.
Which of the following is the disadvantage of
nitrogen, which can be used as carrier gas in gas chromatography?
a) Dangerous to use
b) Expensive
c) Reduced sensitivity
d) High density
8.
What must be done to the solid samples for it
to be introduced into the column without using solid injection syringes in gas
chromatography?
a) Introduced in hot-zone of the column
b) Dissolved in volatile liquids
c) Introduced using rotary sample valve
d) Introduced using sampling loops
9.
Which of the following is the commonly used
support material for the packed column in gas chromatography?
a) Glass
b) Metal
c) Diatomaceous earth
d) Stainless steel
10. Which
of the following is the advantage of a straight packed column?
a) It can be packed uniformly
b) It can be repacked easily
c) It is compact
d) It is easier to heat it evenly
11. Which
of the following is the disadvantage of coiled or helical shaped packed chromatographic column?
a) It cannot be packed uniformly
b) It cannot be repacked easily
c) It is not compact
d) It is not easy to heat it evenly
12. Capillary
columns are open tubular columns constructed from which of the following materials?
a) Glass
b) Metal
c) Stainless steel
d) Fused silica
13. Sample
injection port must be maintained at a temperature at which rapid vapourisation
occurs but thermal degradation does not occur.
a) True
b) False
14. Which
of the following is not a desirable feature of the ovens used in gas
chromatography?
a) It must have a fast rate of heating
b) Power consumption should be kept low
c) It must have maximum thermal gradients
d) It should have proper insulation
15. Which of the following
is not a feature of carrier gas used in gas chromatography?
a) It must be chemically inert
b) It should be suitable for the detector employed
c) It should not be
completely pure :
d) It should be cheap
16. In which of the following
methods are liquid samples injected into the column in gas chromatography?
a) Gas tight syringe
b) Micro-syringe:
c) Rotary sample valve
d) Solid injection syringes
17. Which of the following is the commonly used support
material for the packed column in gas chromatography?
a) Glass
b) Metal
c) Diatomaceous earth:
d) Stainless steel
18. Which of the following is the disadvantage of coiled or
helical shaped packed chromatographic column?
a) It cannot be packed uniformly
b) It cannot be repacked
easily:
c) It is not compact
d) It is not easy to heat it evenly
19. Which detector is not
used in GC ?
a.
FID
b.
TCD
c.
c.A &B
d.
d. TMD
20.
which is not
application of GC chromatography?
a) Quality control and
analysis of drug products like anti biotics (penicilin), anti viral (amantidine),
general anesthetics, etc.
b) polluent like
formaldehyde, carbon monoxide , benzene , DDT etc.
c) ASSAY of drugs – purity
of a compound can be determined for drugs like :-ATROPINE SULPHATE
d)
d)It is purified water.
21. Which
method is used to separation of thermally stable and volatile substance ?
a)
NMR
b)
Mass
sepctroscopy
c)
HPLC
d)
Gas Chromatography.
22. Which
sentence is true about gas Chromatography ?
a)
It
has gaseous stationary phase.
b)
It
is not used to volatile substance.
c)
It is work on principal of the
adsorption and partitions.
d)
When
stationary phase is liquid it is called a GSC
23. Which
liquid are used in GLC ?
a)
Kiesulghur
b)
Diatomaceous
earth
c)
A and B
d)
None
of the above
24. Which
solid are used in GSC ?
a)
Alumina
b)
Carbon
c)
Granular
silica
d) All
of the above
25. In
GLC, which condition is not suitable for stationary phase?
a)
Low
vapour pressure
b)
Non
volatile
c)
A
and B
d) None
of the above
26. Which
of the following is not used for detection in GC?
a) Infrared spectroscopy
b)
NMR
c) Flame Ionization
d) Electrical Conductivity
27. Which
of these effects result from slow injection of a large sample volume?
a) Increased
resolution
b)
Decreased resolution
c) Non-linear
detector response
d) Constant
resolution
28. The GC trace obtained after an
experiment is called as
a) Chromatograph
b)
Chromatogram
c) Chromatophore
d) Graph
29. Which of the following detector give
Concentration-dependent signals?
a) Eletron-capture
detector
b) Thermal
conductivity
c) Infra-red
detector
d)
All of these
30. What is useful information can be found
from a Van Deempter plot?
a) The
selectivity factor
b)
Optimum mobile phase flow
rate
c) Optimum
Colum temperature
d) Optimum
Colum Length
31. What is the typical internal diameter
of fused silica capillary columns?
a)
0.2 to 0.3mm
b) 0.3
to 0.5mm
c) 0.5
to 1.0mm
d) 1.0
to 2.0mm
32. Resolution is proportional to the
a) Number
of the theoretical plates in a column
b)
Square root of number of
theoretical plates in a column
c) Square
of number of theoretical plates in a column
d) Cube
root of the number of theoretical plates in a column
33. Derivatisation of a sample is carried out
to
a) Reduce
polarity of the analytes
b) Increase
the detector response
c) Increase
volatility of the analytes
d) All of the above
34. Which statement is correct?
a) Gas
chromatography is used to analyse gases
b) Gas
chromatography is used to analyse solids
c) Gas
chromatography is used to analyse gases, solutions and solids
d)
All of the above
35. In column switching chromatography
a)
Compounds trapped on one
column are eluted to another column
b) One
column is removed and replaced and replaced by another
c) The
flow to the column is switched on and off repeatedly
d) Any
of the above
36. A retention gap is placed between the
injector and the front of the column to
a)
Retain contaminants and
prevent them from reaching the column
b) Retain
the sample and release it gradually to the column
c) Prevent
backflush of the injected solution
d) All
of the above
37. Which of the following detectors give
mass flow-dependent signals?
a) Electron
capture detector
b)
Field ionization detector
c) Thermal
conductivity detector
d) All
of the above
38. Headspace analysis is carried out in
order to
a)
Analyse volatile
compounds from solid or liquid samples
b) Determine
the psychological state of the tutor
c) Analyse
the column contents ahead to the sample
d) Determine
non-volatile
39. Split injection is carried out by
a) Splitting
the sample into smaller portions to inject sequentially
b) Splitting
the sample into smaller portions to inject at the same time through parallel
ports
c)
Splitting off some of the
sample so that it does not enter the
column
d) None
of the above
40. What does the retention factor, k,
describe?
a) The
distribution of an analyse between the stationary and the mobile phase
b)
The migration rate of an analyte
though a column
c) The
velocity of the mobile phase
d) All
of the above
41. Theoretical plates are used to
a)
Estimate the efficiency of
a column
b) Determine
the thickness of the stationary phase
c) Measure
the distribution of the analyte between mobile and stationary phases
d) None
of the above
42. What the selectivity factor describe
a) The
proportional difference in widths of two chromatographic peaks
b) The
maximum number of different species which a column can separate simultaneously
c)
The relative separation
achieved between two species
d) None
of the above
43. Helium is generally preffered as a
carrier gas over nitrogen and hydrogen because
a) It is
inert
b) It has
a lower viscocity
c) It doubles
up as a party gas for balloons and funny voices
d) All of the above
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