Hello friends, in today's article we see the MCQ's of sterilization in microbiology. So let's see one by one mcq's of sterilization in microbiology.
MCQ's of Sterilization in microbiology
MCQ’s
of Sterilization in microbiology
1. The medium used in membrane
filter technique was
a. EMB agar
b.
EMR-Vp medium
c. Lactose
broth
d. Endo agar
2. Lysol is a
a. Sterilent
b. Disinfectant
c. Antiseptic
d. Antifungal agent
3. Which of the following is a neutral stain?
a. Picric acid
b. Gmiemsa
c. Neutral red
d. Malachite green
4. Peptone water medium is an example for
a.
Synthetic medium
b. Semisynthetic medium
c. Differential
medium
d.
None of these
5. The method in which the cells are frozen
dehydrated is called
a. Pasteurization
b. Dessication
c. Disinfection
d. Lypophilization
6. The technique used to avoid all microorganisms is accomplished by
a. Sterlization
b.
Disinfection
c. Surgical sterilization
d.
Disinfection Sterilization
7. Thermal death time is
a. Time required
to kill all cells at a given
temperature
b. Temperature that kills
all cells in a given
time
c. Time and temperature needed to kill all cells
d.
All of
the above
8. A culture medium the exact composition of which is not known was called as
a. Simple
b.
Complex
c. Defined
d. Natural
9. Elek’s gel diffusion test is used for the detection of
a.
Tetani toxin
b.
Cholera toxin
c.
Diophtheria toxin
d. Toxoid
10. Temperature required for pasteurization is
a.
Above 150oC
b. Below 100oC
c. 110oC
d. None of these
11. Separation
of a single bacterial colony
is calle
a. Isolation
b.
Separation
c. Pure culturing
d. All of these
12. Which of the following is ionizing radiation?
a. U.V. rays
b. IR
c. gama-rays
d. None of these
13. Which of the following induces dimerisation of thymine?
a. X-rays
b. U.V. rays
c. ã-rays
d. None of these
14. When food material are preserved at a temperature just above freezing
temperature, the process is called.
a. Freezing
b. Pasteurisation
c. Chilling
d. Frosting
15. Which of the following method of sterilization has no effect on spores?
a.
Drying
b. b. Hot air
oven
c. Autoclave
d. None of these
16. Treponema pallidum can be best indentified
using
a.
Fluorescence microscope
b. Bright field microscope
c. Dark field microscope
d.
Flourescence microscope
17. Autoclaving is carried at
a.
Dry heat
b. Atmospheric pressure
c. 120oC
d. All of these
18. Temperature in pasteurization is
a.
62.8oC
b.
35.7oC
c. 68.2oC
d.
60.8oC
19. The bacterial culture prepared by pure culture method is
a.
Inoculum
b. Suspension
c. Dilution
d. None of these
20. Algae are rich in
a. Carbohydrates
b. Proteins
c. Vitamins
d. All of these
21. L-Lysine is produced from
a. Corynebacterium glutamicum
b. Clostridium botulinum
c. Mycobacterium sps
d.
Pseudomonas
22. The orderly increase in the quantity of all of the cellular components is known as
a.
Reproduction
b. Growth
c. Binary
fission
d. None of these
23. Theobacillus thio oxidans grow at pH
a. 7.0
b. 1.0
c. 6.0
d. 9.5
24. Slow freezing requires the conditions
a. 0oC to 15oC for 15 min.
b.
– 6 oC to – 10oC for 10 min.
c.
– 15oC to 3 to 72 hrs.
d. None of these
25. Discontinuous heating is called
a.
Pasteurization
b.
Sterilization
c. Fermentation
d. Tindalisation
26. Isolation is
a.
Purification
of culture
b.
Introduction
of inoculum
c.
Separation of a single colony
d. To grow microorganisms on surfaces
27. The condition required for autoclave
a. 121oC temp.and
15 lbs. pressure
for 20 min.
b. 120oC temp.and 20 lbs. pressure for 30 min
c.
150oC temp. for 1 hr.
d. 130oC temp for
2 hr.
28. Lysozyme is effective against
a.
Gram
negative bacteria
b. Gram
positive bacteria
c. Protozoa
d.
Helminthes
29. Blood agar medium is
a.
Enrichment medium
b. Enriched medium
c. Selective medium
d.
Differential medium
30. Infrared radiation is a method of steril- ization by
a.
Dry heat
b.
Moist heat
c. Chemical method
d.
Mechanical method
31. Lyophilization means
a.
Sterilization
b.
Freeze-drying
c. Burning to
ashes
d.
Exposure
to formation
32. Temperature used for hot air oven is
a.
100oC for 1 hour
b.
120oC for 1 hour
c.
160oC
for 1 hour
d. 60oC
for 1 hour
33. Phenol co-efficient indicates
a. Efficiency
of a disinfectant
b.
Dilution
of a disinfectant
c. Purity of a
disinfectant
d.
Quantity
of a disinfectant
34. This is an agar plate method and is commonly used for estimation of the number of bacteria in milk.
a. Standard
Plate Count (SPC)
b.
Spread plate
c. Lawn culture
d.
Roll
tube method
35.
Agar
is obtained form
a. Brown algae
b.
Red algae
c. Green algae
d. Blue-green algae
36.
A gram positive organism which produces
swarming on culture medium is
a. Salmonella
b. Clostridium
c. Staphylococci
d. Proteus
37. Enhancement of virulence in bacteria is known as
a. Pathogenicity
b. Attenuation
c. Exaltation
d. Toxigenicity
38. For effective sterilization in an autoclave the temperature obtained is
a. 50oC
b. 100oC
c. 120oC
d. 180oC
39. Spores are killed by
a. 70% alcohol
b. Glutaraldehyde
c. Autoclaving
d. Both b and c
40. Glassware are sterilized by
a. Autoclaving
b.
Hot air over
c. Incineration
d. None of these
41. Tyndallisation was proposed by
a.
Tyndall
b. Pasteur
c. Koch
d. Jenner
42. Viruses can be cultivated in
a. Lab media
b. Broth
c. Living cells
d. None of these
43. By pasteurization
a.
All the microorganisms can be removed
b. Only pathogenic forms can be removed
c. Only non-pathogenic forms can be removed
d.
All of
these are correct
44.
The
temperature required for pasteuriza- tion is
a. Above 100oC
b.
Below 100oC
c. 100oC
d. None of these
45.
In the medium other
than nutrients, if any substance is used in excess, that
medium is
a. Enriched medium
b. Special medium
c. Enrichment medium
d. None of these
46. Example for indicator medium is
a.
Nutrient Agar
b. Nutrient broth
c. Wilson and Blair
d. Czapeck-dox medium
47. Example of Anaerobic medium is
a. Robertson
cooked-meat medium
b. Nutrient agar
c. Nutrient broth
d.
Mac-Conkey’s agar
48.
The
differentiate lactose and non-lactose fermentors, the medium used is
a.
Wilson
& lair
b. Blood Agar
c. Tetra thionate broth
d. Mac-Conkey’s Agar
49. Best method for getting pure culture is
a. Streak-plate
b. Agar slant
c. Both a & b
d. None of these
50. To transfer cultures from one place to another,
the device used is
a. Slant
b.
Needle
c. Inoculation loop
d. Autoclave
51. The bacterial culture prepared by pure culture is
a.
Inoculum
b. Suspension
c. Dilution
d. None of these
52. Separation of a single colony is
a. Pure-culturing
b. Isolation
c. Separation
d. Both a and b
53. Growth period of the culture is
a. Inoculation
b. . Incubation
c. Incineration
d. Isolation
54. At the temperature 160oC for one hour, complete
sterilization occurs in
a. Autoclave
b. Hot air oven
c. Laminar flow
d. Incubator
55. In autoclave, the principle involved is
a.
Dry heat
b. Moist heat
c. Steam under pressur
d. Both b
and c
56. The spores of th bacteria which can withstand the moist heat effect also
a.
Bacillus
subtilis
b. Coxiella burnetti
c. Bacillus stearothermophilus
d.
Pseudomonas
57. Factors on which disinfectivity of a disinfectant depends
a.
Concentration
of the substance
b. Time of action
c. pH of the medium and temperature suitable for the chemical
d. All of
the above
58.
Aldehydes, which are most powerful disinfectants
a. Formaldehyde
b. . Acetaldehyde
c. Glutamal aldehyde
d.
Both a and c
59. Accridine
dyes are more
effective against
a. Gram positive
b.
Gram negative
c. Mycoplasmas
d. Rickttsiae
60. The sterilizing agent is
a.
Ethelene oxide
b. Oxygen
c. Nitrogen
d. Carbon tetrachloride
61.
Salts
of heavy metals used as disinfec- tants are
a. Thiomersal
b. Phenyl mercury nitrate
c. Mercurochrome
d. All of these
62.
Cultures are prepared by penetrating the inoculation loop
with suspension into the
medium, they are
a. Stock cultures
b.
Stabcultures
c. Sub-cultures
d. None of these
63.
The
principle involved in the streak plate method
is
a.
Separation
b.
Streaking
c. Isolation
d. Dilution
64. Culture media for fungi are
a.
Potato
dextrose agar (PDA)
b.
Sabouraud’s agar
c. Czapekdox
agar
d. All of
the above
65.
Spores of actinomycetes are very sensitive, killed at room temperature of
a. 52oC for 30 min.
b.
65oC
for 30 min.
c. 70oC for 30 min.
d. 43oC for 30 min.
66.
The
term that is used for the bacteria which can withstand pasteurization but does
not grow at higher temperatures
a.
Thermophiles
b.
Extreme thermophiles
c.
Thermoduric
d.
Facultative thermophiles
67. A common laboratory method of cultivating anaerobic micro-organisms is
a.
Gas
pack system
b. Brewer jar system
c. Pyrogallic acid over the cotton
d.
None of these
68. Alkaliphiles grow at pH value between
a. 1 to 6
b. 6 to 9
c. 1 to 11
d. 7 to 12
69. The micro-organisms grow at high salinity are
a. Osmophiles
b. Halophiles
c. Both a and b
d. None of these
70. Non-lactose
fermenting colonies seen on
Mac Conkey’s medium are
a. Salmonella typhi
b. Escherichia coli
c. Klebsiella pneumoniae
d.
Shigella shigae
71. Wilson and Blair medium is used for isolation of
a. Staphylococci
b. Salmonella typhosa
c. Vibrio cholerae
d. Shigella shigae
72. Laboratory diagnosis of enteric fever is
based on
a. Blood culture
b. Urine and stool culture
c. Widal test
d. All of
the above
73. Shigella was first isolated by
a. Shiga
b. Schmitz
c. Sonnei
d. Robert Koch
74. Which of the following are gas producing Salmonella?
a. S.typhi
b.
S.enteritidis
c. S.cholerasuis
d. S.typhimurium
75. Kauffmann white scheme is used to detect
a.
Salmonella spp.
b. Shigella spp.
c. E.coli
d. None of these
76. On Mac Conkey’s medium
Esch. Coli forms
a.
Colorless colonies
b. Greenish pigmentation
c. Pink coloured colonies
d.
Medusa
head appearance
77. C.diphtheriae requires
a.
LJ medium
b. Mac Conkey’s medium
c. Potassium tellurite medium
d.
PDA medium
78. Culture medium for Mycobacterium tuberculosis
a. L J medium
b. Mac Conkey’s medium
c. Wilson blair medium
d. None of these
79. Lepra bacillus is best cultured on
a.
Armadillo’s brain
b. Foot pad of mice
c. Liver of guinea pig
d.
Any of
the above
80. Culture medium for clostridia spp.
a.
76 Lower
stein Jensen’s medium
b. Mac Conkey’s medium
c. Robertson’s cooked meat medium
d. None of these
81. Clsotridium welchii is positive for
a.
Elek’s
gel precipitation test
b. Nagler’s test
c. Weil felix test
d.
Bacitracin
test
82. Nagler’s reaction detects
a.
Coagulase
b. Hyaluronidase
c. Lecithinase
d. None of these
83. Incubation period of Cl. welchii is
a. 8-12 hours
b. 7-10 hours
c. 5-7 hours
d. 2-4 hours
84. The average incubation period of tetanus is
a.
2-3 days
b. 7-10 days
c. 14-21 days
d. 3-4 weeks
85. Salt agar is used for
a. Streptococcus
b. Staphylococcus
c. Vibrio
d. Shigella
86. Culture medium of Leishmania is
a.
Sabousand’s medium
b. NNN medium
c. Wilson Blair medium
d. Czapek – dox medium
87. A simple asexual spore which develops by
budding is known as
a. Chlamydospore
b. Blastospore
c. Arthospore
d. Conidia
88. Culture medium used for fungus is
a.
Sabouraud’s medium
b. Nutrient agar
c. Nutrient broth
d.
Minimal
agar medium
89.
For sterilization of fermentation equipment the method followed is
a. Radiation
b. Chemicals
c. Heating
d. All of these
90. Listed below are substances which are
assayed by organisms mentioned in A to
E. Match them correctly:
1.
Crystal Violet
I.P. A.
Pasteurella pestis
2.
Ampicillin I.P. B. Bacillus cerus
3.
Plaque Vaccine I.P.
C. Micrococcus luteus
4.
Rifampicin D. Lactobacillus aureus
E.
Lactobacillus aureus
F.
Bacillus subtillus
G. Answer:
|
90. 1.d, 2.c, |
3.a, 4.e |
91. Match the following terms with their respective formulations A to E:
1.
Lysol A. Higher boiling fractions of the tar acids
2.
Black fluids B. Prepared from refined tar acids
3.
White fluids C. Solution
of cresol with
soap
4.
Iodophores D. Basic molecules has varying
numbers
of amino groups
E. Iodine
combined with complex
organic chemicals
Answer:
|
91. 1.c, 2.a, |
3.b, 4.e |
92.
Match the following tests with their respective applications A to E:
1.
Schick test A. Tuberculosis
2.
Mantoux test B. Detection
of extraneous
microorganisms
3.
Sterility test C.
Diphtheria toxin
4.
Potency test D. Detection of infection caused
by Rickettsia prowazeki
E. Usefulness of immunological products
Answer:
|
92. 1.c, 2.a, |
3.b, 4.e |
93.
Match the following equipments with their respective methods of sterilization A to E:
1.
Glass syringes A. Autoclave
2.
Disposable B. Chemical instrument
3.
Respiratory parts C. Dry heat
4.
Dialysis machine D.
g-Radiation
E.
Chicken pox in children
Answer:
|
93. 1.c,
2.d, |
3.e, 4.b |
94.
The items listed from A to D can be identified
by the tests given below :
1.
Coomb’s test A. Candida
albicans
2.
Coagulase test B.
Virulent staphylococcus aureus
C. Mycobacterium tuberculosis
D. Non-agglutinating antibodies
Answer: 94. 1.d, 2.a
95. D.pneumoniae can be cultivated in
a.
Glucose broth
b. Serum broth
c. Agar and blood agar
d. Chocolate agar
e. All of these
96. D.pneumoniae can be identified by
a.
Microscopic exam
b. Culture of sputum/blood
c. Animal inoculation
d. All of these
e. None of these
97.
The
diagnosis of tuberculosis is carried out by
a.
Emulator
b.
Antiformin method
c. Petroff’s method
d. Concentration method
e. All
of these
98. The size of the virus can be determined by
a.
Micrography
b. Ultra-centrifugation at high speed
c. Ultra-filteration
d. All of these
99.
Differential
staining of bacteria spore is related to
a.
Albert’s staining
b. Lugol’ s staining
c. Moller’s staining
d. Indian ink preparation
100.
Electron microscope studies does not help in identifying the section of bacterial spore
a.
Core
b.
b.
Spore cortex
c. Capsule
d. All of these
101.
Wilson and Blair bismuth sulphite medium
is used for the growth
a. Salmonella typhi
b. Shigella dysenteriae
c. Vibrio cholerae
d. E. coli
102.
Which
Rickettsia can be grown on blood agar media?
a. Lactobacilli
b.
Streptobacillus
c. Bacillus
anthrax
d. Vibrio cholerae
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