Paper Chromatography Technique in biology MCQs.

 Hello friends in todays article we see the paper chromatography MCQs, so let's begin

Paper Chromatography Technique in Biology

1.      When there are to complex mixture are separated which development technique of paper Chromatography is used ?

A.    Ascending

B.     Descending

C.     Radiation

D.    Two dimensional

2.      How much time is required to saturate of chamber ?

A.    12 hour

B.     24 hour

C.     6 hour

D.    10 hour

3.      Which force is responsible for the separation of the components in descending paper chromatography?

A.    Partition

B.     Adsorption

C.     Gravity

D.    All of the above

4.      Which is not development technique of paper Chromatography ?

A.    Two dimensional

B.     Ascending

C.     Descending

D.    HPLC

5.      What is the principle of the paper chromatography ?

A.    Partition

B.     Adsorption

C.     A and B

D.    None of the above

6.      Rf value is

A.    Distance travelled by the compound at it’s point of maximum.

B.     Distance travelled by the standard.

C.     Solvent travelled

D.    None of the above

7.      Which type of filter paper are mostly used in paper chromatography?

A.    Butter paper

B.     Sample paper

C.     Whatmann filter paper

D.    Filter paper

8.      Which factor is not affect in stationary phases in paper chromatography ?

A.    Thickness

B.     Flow rate

C.     Purity

D.    Freezing point

9.      Size of spot in paper chromatography is

A.    2-5 mm

B.     1-2 mm

C.     7-8 mm

D.    6-8 mm

10.  What is the ratio of  Isopropanol: Ammonia: water in mobile phase?

A.    5:3:1

B.     6:7:8

C.     9:1:2

D.    3:3:6

11.  How many  percentage of the Beta cellulose in stationary phase?

A.    0.3-1%

B.     2-4%

C.     3-7%

D.    2-4%

12.  Chromatography is a physical method that is used to separate and analyse __________
a) Simple mixtures
b) Complex mixtures
c) Viscous mixtures
d) Metals

13.  In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?
a) Column chromatography
b) Planar chromatography
c) Liquid chromatography
d) Gas chromatography

14.  In chromatography, the stationary phase can be ___________ supported on a solid.
a) Solid or liquid
b) Liquid or gas
c) Solid only
d) Liquid only

15.  In chromatography, which of the following can the mobile phase be made of?
a) Solid or liquid
b) Liquid or gas
c) Gas only
d) Liquid only

16.  Which of the following cannot be used as an adsorbent in Column adsorption chromatography?
a) Magnesium oxide
b) Silica gel
c) Activated alumina
d) Potassium permanganate

17.  Which of the following types of chromatography involves the separation of substances in a mixture over a 0.2mm thick layer of an adsorbent?
a) Gas liquid
b) Column
c) Thin layer
d) Paper

18.  Chromatography cannot be used to purify volatile substances.
a) True
b) False

19.  In Column chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

20.  Chromatography cannot be used to separate delicate products.
a) True
b) False

21.  In Thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

22.  In which of the following type of paper, chromatography does the mobile phase move horizontally over a circular sheet of paper?
a) Ascending paper chromatography
b) Descending paper chromatography
c) Radial paper chromatography
d) Ascending – descending chromatography

23.  Liquid chromatography can be performed in which of the following ways?
a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces

24.  Gas chromatography can be performed in which of the following ways?

a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces

25.  Which of the following types of chromatography involves the process, where the mobile phase moves through the stationary phase by the influence of gravity or capillary action?
a) Column Chromatography
b) High Pressure Liquid Chromatography
c) Gas Chromatography
d) Planar Chromatography

26.   Chromatography is a physical method that is used to separate and analyse __________

a) Simple mixtures
b) Complex mixtures
c) Viscous mixtures
d) Metals

27.  In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

a) Column chromatography
b) Planar chromatography
c) Liquid chromatography
d) Gas chromatography

28.  Chromatograhy can be used

a)      Form mistures

b)      Change mixture compositions

c)      Separate mixture into pure substances

d)     None of the above

29.  The phase that never gains in sublimation is

a)      Solid

b)      Liquid

c)      Gas

d)     Vapours

30.  Chromatography refers to the

a)      Concentration of chromium in a substance

b)      Mass of chromium

c)      Testing of additives

d)     Testing of the alkalinity or acidity

31.  Locating agent of amino acids is

a)      Diazo reagent

b)      Ninhydrin spray

c)      Amphoteric oxides

d)     Neutral oxides

32.  Chromatography is especially useful for

a)      Food only

b)      Drugs only

c)      Salt solutions

d)     Food and drugs

33,   Which type of m.p. are used in paper chromatography ?

A.    The mobile phase which gives Rf value range between 0.1-0.3

B.     The mobile phase which gives Rf value range between 0.2-0.8

C.     The mobile phase which gives Rf value range between 0.8-1

D.    D.The mobile phase which gives Rf value range between 0.6.