Hello friends, in today's article, we see the MCQs of Labeling of DNA and RNA. In this, we see the DNA manipulation MCQs, DNA labeling MCQs, and RNA MCQs.
so let's start one by one
Labeling of DNA and RNA MCQs:-
Labeling of DNA and RNA MCQs:-
1. Which of the following does not affect the formation of hybrid DNA?
a) Ionic strength
b) Pressure
c) Temperature
d) Homologous DNA
2. Which of the following is not termed hybridization?
a) DNA and cDNA
b) DNA and mRNA
c) DNA from different species
d) DNA from males and females of the same species
3. What is a probe?
a) Chemically synthesized DNA
b) Purified DNA
c) Fragmented DNA duplex
d) Either purified or synthesized single-stranded DNA
4. Why is a probe labeled?
a) Improve visibility
b) Improve stability
c) Improve location identification
d) Improve binding capability
5. In how many ways DNA can be labeled?
a) 2
b) 3
c) 4
d) 5
6. What is the function of polynucleotide kinase?
a) Addition of γ – phosphate at 3’ – OH
b) Addition of γ – phosphate at 5’ – OH
c) Removal of γ – phosphate at 3’ – OH
d) Removal of γ – phosphate at 5’ – OH
7. Fluorescent labeling is an important phenomenon in the case of hybridization and manipulations in molecular biology.
a) True
b) False
8. Radiolabelling is generally brought about by the addition of radioactive phosphorus.
a) True
b) False
9. During electrophoresis denaturation of the double-stranded DNA is brought about by __________
a) Treatment with alkali
b) Application of current
c) Treatment with EtBr
d) Application of heat
10. In the case of northern blotting the hybridization occurs between two RNA molecules.
a) True
b) False
(Labeling of DNA and RNA MCQs most probably ask in CSIR NET Exam.)
11. Northern blotting is performed for ____________
a) Determining the size of DNA
b) Determining the size of RNA
c) Quantification of RNA
d) Sequencing of RNA
12. The process by which a probe is used to screen a library is known as ___________
a) Hybridization
b) Southern blotting
c) Colony hybridization
d) Western blotting
13. During DNA cloning which of the following is not a crucial requirement?
a) DNA inserts
b) Vector
c) Protein expression
d) Molecular cutter
14. Transformation does not involve ____________
a) Cutting
b) Recombination
c) Propagation
d) Expression
15. Which is the most common organism considered for genetic manipulations?
a) E. coli
b) Saccharomyces cerevisiae
c) Cyanobacteria
d) Bacillus Subtilis
16. Which of the following is an essential feature for being a perfect vector?
a) Origin of replication
b) Selectable marker
c) Restriction site
d) Virulent gene
17. DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme.
a) True
b) False
(Labeling of DNA and RNA MCQs most probably ask in CSIR NET Exam.)
18. The vector and the DNA insert are cut by different enzymes for convenience.
a) True
b) False
19. What is the major difference between cloning vectors and primary vectors?
a) Selectable marker
b) DNA inserts
c) Presence of promoter
d) Presence of two Ori
20. Which of the following is the primary use of an expression vector?
a) DNA library
b) DNA purification
c) Protein production
d) DNA cloning
21. Under the influence of which ion does E. coli take up plasmid from the environment?
a) Nickel
b) Copper
c) Lead
d) Sulphur
22. The process by which every type of transformant can be identified is __________
a) Replica plating
b) Hybridization
c) Blotting
d) Insertional inactivation
23. Many cells have a cell wall, but animal cells do not. Why might this be?
a) Because animal cells only exist in the multicellular form
b) Because animal cells do not need structural support
c) Because animal cells are capable of movement
d) Because animal cells can control osmotic pressure
24. In terms of DNA and RNA structure, what is a nucleotide?
a) A nucleotide is a heterocyclic base
b) A nucleotide is a sugar molecule covalently bonded to a heterocyclic base
c) A nucleotide is a sugar molecule bonded to phosphate group/s and a heterocyclic base
d) A nucleotide is a heterocyclic base bonded to phosphate group/s
25. DNA exists in a double-stranded form whereas RNA is mainly a single-stranded molecule. What is the likely reason for DNA being double-stranded?
a) RNA strands cannot form base pairs
b) Double-stranded DNA is a more stable structure
c) DNA cannot exist in the single-stranded form
d) It is easier to replicate double-stranded DNA than single-stranded RNA
26. In DNA tertiary structure, what is a histone octamer?
a) A complex consisting of eight positively charged histone proteins (two of each H2A, H2B, H3, and H4) that aid in the packaging of DNA
b) A complex consisting of eight negatively charged histone proteins (two of each H2A, H2B, H3, and H4) that aid in the packaging of DNA
c) A complex consisting of nine positively charged histone proteins (H1 and two of each H2A, H2B, H3, and H4) that aid in the packaging of DNA.
d) A complex consisting of nine negatively charged histone proteins (H1 and two of each H2A, H2B, H3, and H4) that aid in the packaging of DNA.
27. How many stop codons are there in the genetic code?
a) 1
b) 2
c) 3
d) 4
(Labeling of DNA and RNA MCQs most probably ask in CSIR NET Exam.)
28. Which of the following statements is true about DNA polymerase?
a) DNA polymerase can synthesize mRNA in the 3' to 5' direction
b) DNA polymerase can synthesize DNA in the 5' to 3' direction
c) DNA polymerase can synthesize mRNA in the 5' to 3'direction
d) DNA polymerase can synthesize DNA in the 3' to 5' direction
29. What is the role of topoisomerases in eukaryotic DNA replication?
a) Topoisomerase enzymes cut, uncoil and reseal the double-stranded DNA
b) Topoisomerase enzymes bind to the origin of replication sites within double-stranded DNA
c) Topoisomerase enzymes open up the double-stranded DNA at the replication fork
d) Topoisomerase enzymes join the Okazaki fragments together with phosphodiester bonds
30. Which of the following statements is true of DNA damage?
a) All DNA damage results in diseases such as cancer
b) Most DNA damage is repaired by the cell
c) All DNA damage is caused by physical, chemical, or biological agents
d) Most DNA damage is advantageous to the cell
31. Why are genetic disorders such as hemophilia and Duchenne muscular dystrophy more prevalent in males than females?
a) Because they can only be passed on from father to son
b) Because they are dominant genetic disorders
c) Because they occur due to spontaneous mutations in the Y-chromosome
d) Because they are X-linked recessive disorders
32. Which of the following is not a researched means of delivering therapeutic DNA for gene therapy?
a) Polymers
b) Liposomes
c) Bacteria
d) Viruses
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Answer:-
1) B
2) D
3) D
4) C
5) A
6) B
7) B
8) A
9) A
10) B
11) C
12) C
13) C
14) D
15) A
16) D
17) A
18) B
19) C
20) C
21) B
22) D
23) D
24) C
25) B
26) A
27) C
28) B
29) A
30) B
31) D
32) C
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