Northern and Western Blotting Technique MCQs most probably ask in CSIR NET Exam

 Hello friends, in today's article we see the northern and western blotting technique MCQs, which most probably ask in CSIR NET exam.

so let's see one by one

Northern and Western Blotting Technique MCQs:-

Northern and Western blotting MCQs

1. Western blotting is the technique for the detection of 

1. Specific DNA in Sample

2. Specific RNA in a sample

3. Specific protein in a sample

4. Specific glycolipid in a sample

2. Arrange the following in a correct order

1. Southern Blotting ……. A) Alwine

2. Western Blotting ………. B) E.M. Southern

3. Northern blotting ……….. C) A. Jeffrey

4. DNA fingerprinting …….. D) Towbin

1. 1-A, 2-C, 3-D, 4-B

2. 1-B, 2-D, 3-A, 4-C

3. 1-B, 2-A, 3-D, 4-C

4. 1-B, 2-C, 3-A, 4-C

3. Labelled antibodies are used to detect

1. Detect the presence of a particular DNA molecule in southern blotting

2. Detect the presence of a particular RNA molecule in southern blotting

3. Detect the presence of a particular protein molecule in southern blotting

4. Detect the presence of a particular protein molecule in western blotting

4. Which of the following technique is suitable for identifying mRNA molecules in a Sample

1. Western blotting

2. Southern blotting

3. Northern blotting

4. Eastern Blotting

5. Which of the following technique doesn’t involve electrophoresis for the separation of biomolecules

1. Dot blotting

2. Southern blotting

3. Northern blotting

4. Western blotting

6. Probe is a

1. Protein for detecting specific DNA molecules

2. A short piece of labeled DNA Which are complementary to the nucleic acid strand to be detected

3. A short piece of labeled DNA or RNA which are complementary to the nucleic acid strand to be detected

4. None of these

 

7. Which of the following technique is most suitable for detecting the presence of a gene product?

1. Dot blotting

2. Southern blotting

3. Plaque blotting

4. Western blotting

8. Aminobenzyloxymetheyl filter paper is commonly used for transfer in 

1. Western blotting

2. Southern blotting

3. Northern blotting

4. Dot blotting

9. Arrange the following in the correct order

1. Southern blotting……….A) RNA-DNA hybrid

2. Western blotting………...B) DNA-DNA hybrid

3. Northern blotting………..C) Southern blotting

4. DNA fingerprinting………D) antigen-antibody reaction

10. Which of the following is used to lyse the nucleus and release the DNA?

a) sodium dodecyl sulfate

b) ammonium sulfate

c) ferric phosphate

d) fluorine 

11. In purification steps of nucleic-acids, phenol is used for _______________________

a) deproteinization

b) denaturation

c) lowering pH

d) lowering viscosity

12. Which of the following is used to precipitate the nucleic acids?

a) glycerol

b) ethanol

c) methanol

d) ethylene

13. Phenol is an active protein denaturant.

a) True

b) False

14. Which type of gel is used for large nucleic acids?

a) acrylamide

b) cellulose

c) agarose

d) Sephadex

15. Which technique is used to separate nucleic acids of size greater than 25 kb?

a) SDS-PAGE

b) Pulsed-field electrophoresis

c) 2D- gel electrophoresis

d) Isoelectric focusing

16. One Svedberg unit is equivalent to 10-13 ______________

a) hours

b) minutes

c) seconds

d) milli-seconds

17. In rate-zonal sedimentation, the nucleic acids are separated base on __________________

a) nucleotide length

b) relative charge

c) relative mass

d) mass-to-charge ratio

18. Equilibrium centrifugation is also known as ___________________ centrifugation.

a) isotonic

b) isopycnic

c) gradient

d) sucrose

19. Which technique can be used to separate 15N and 14N?

a) Rate-zonal centrifugation

b) Isopycnic centrifugation

c) HPLC

d) Mass spectrometry

20. Nucleic acid hybridization is used to identify ________________________

a) RNAs

b) DNAs

c) Complementary base sequences

d) Proteins

21. The procedure by which DNA gel is transferred to a membrane is termed as ___________________

a) blotting

b) centrifugation

c) electrophoresis

d) paper chromatography

22. In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?

a) Western blotting

b) Southern blotting

c) Isopycnic centrifugation

d) Spectrophotometry

23. Which of the following molecules can be analyzed using a northern blot?

a) RNA

b) Carbohydrates

c) Proteins

d) DNA

24. Which of the following is a commonly used label in blotting techniques?

a) vimentin

b) biotin

c) avidin

d) streptomycin

25. Northern blot is used for the separation of:

a) mRNA

b) DNA

c) Protein

d) Protein DNA interaction

26. Northern blotting is not a variant of Southern blotting

1. True

2. False

27. Which of the following is found in northern blotting but not in southern blotting?

1. Gel

2. Nitrocellulose

3. Reactive paper

4. Probe

28. The reactive paper used is prepared by which technique?

1. Heating

2. Hydrolysis

3. Acidification

4. Diazotization

29. How can hybridizing bands be located?

1. Radiography

2. Autoradiography

3. UV radiation

4. Infrared radiation

30. When was it found that RNA can be bound to nitrocellulose membrane under appropriate conditions?

1. 1980

2. 1880

3. 1940

4. 1950

31. Nylon membranes have superseded the need for ______ in southern blotting techniques.

1. Probe

2. RNA

3. DBM paper

4. Gel

32. Which of the following techniques does not involve nucleic acids?

1. Northern blotting

2. Western blotting

3. PFGE

4. Southern blotting

33. Western blotting is used to transfer ________

1. Genes

2. Proteins

3. DNA

4. Probes

34. When was the western blotting technique developed?

1. 1980

2. 1961

3. 1981

4. 1971

35. The protein bands transferred by the western blotting are previously _______

1. Electrophoresed 

2. Heated

3. Calibrated 

4. Mixed

36. In western blotting, proteins bind__________ to the membrane.

1. Loosely

2. Covalently

3. Irreversibly

4. strongly

37. Which interactions are made use of in the western blotting technique?

1. Covalent

2. Hydrophobic

3. Protein-ligand

4. Protein-protein

38. Which is the most common ligand in Western blotting?

1. Lactose

2. Toxins

3. Genes

4. Antibodies

39. Lectings identify which of the following in a western blotting technique?

1. Glucoprotein

2. Glycoprotein

3. Antibiotic

4. RNA

40. What are the lectins?

1. Antigen

2. Antibody

3. DNA

4. RNA

Answer:-

1) C

2) B

3) D

4) C

5) A

6) C

7) D

8) C

9) B

10) A

11) A

12) B

13) A

14) C

15) B

16) C

17) A

18) B

19) B

20) C

21) A

22) B

23) A

24) B

25) A

26) B

27) C

28) D

29) B

30) A

31) C

32) B

33) B

34) C

35) A

36) D

37) C

38) D

39) B

40) B